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Image Search Results
Journal: BMC Medical Genetics
Article Title: Suggestive evidence of associations between liver X receptor β polymorphisms with type 2 diabetes mellitus and obesity in three cohort studies: HUNT2 (Norway), MONICA (France) and HELENA (Europe)
doi: 10.1186/1471-2350-11-144
Figure Lengend Snippet: Functional analyses of the rs28514894 SNP in luciferase reporter gene transfections in HepG2 (A) and JEG3 (B) cells . HepG2 and JEG3 cells were transiently co-transfected with either the empty basic-pGL3 (O) or the pGL3-LXRβ*T allele or the pGL3-LXRβ*C allele vector with or without an HNF4α or NF1s expression vector. Activity was expressed as fold activity compared with the pGL3-LXRβ*T vector and the empty basic-pGL3 vector. White and black bars represent activities obtained without or with HNF4α or NF1s, respectively. Each bar represents the means ± SEM of at least 3 independent experiments. Firefly luciferase activities were normalized against renilla luciferase activities. Data were compared using the Kruskal-Wallis non-parametric test. * p < 0.05, ** p < 0.01.
Article Snippet: The
Techniques: Functional Assay, Luciferase, Transfection, Plasmid Preparation, Expressing, Activity Assay